SubtiBank SubtiBank
mrfA [2018-11-01 12:30:43]
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

mrfA [2018-11-01 12:30:43]

ATP-dependent helicase
locus
BSU22220
pI
6.16
mw
84.40 kDa
protein length
749 aa Sequence Blast
gene length
2247 bp Sequence Blast
function
mitomycin C-specific DNA damage repair
product
helicase
essential
no
synonyms
yprA
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination] → [category|SW 3.1.5.6|Other proteins]

    • Gene

    Coordinates
    2,334,581 → 2,336,830

    The protein

    Structure

  • [PDB|2P6R] (the N-terminal domain, corresponds to aa 31 ... 451 of MrfA, 25% identity) [pubmed|17558417]

    • Expression and Regulation

    Operons

    genes
    [gene|4D286C045A43FBF0F6E93E9E46AD7F25E35ADA7C|mrfA]-[gene|3F1D78CAAA4B5057DEBB24C4D382F96DA4AA4852|mrfB]-[gene|FA8D9AA0968365522DEFC05B277123FC96482044|cotD]
    description
    [Pubmed|22383849]
    view in new tab

    Biological materials

    Mutant

  • MGNA-A412 (yprA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/412 NBRP B. subtilis, Japan]
  • BKE22220 (Δ[gene|4D286C045A43FBF0F6E93E9E46AD7F25E35ADA7C|mrfA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE22220 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTTTACACCTCTTTGT, downstream forward: _UP4_ATGTCGTAAGGAGGGAGGCC
  • BKK22220 (Δ[gene|4D286C045A43FBF0F6E93E9E46AD7F25E35ADA7C|mrfA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK22220 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTTTACACCTCTTTGT, downstream forward: _UP4_ATGTCGTAAGGAGGGAGGCC

    • References

    Research papers

  • 30379365,17558417